UV-Vis Spectrophotometry- NanoDrop

About the service
NanoDrop spectrophotometer is renowned for its ability to measure low volumes in the field of spectrophotometry, particularly in biological sciences. Leveraging the innovative "Acclaro smart technology," this device enables quantitative and qualitative measurements of various samples, including RNA, DNA, peptides, and proteins. It can identify sample contamination with just 1-2 microliters of volume in a few seconds and provide results in terms of optical density (OD), concentration, and a comparative chart. Additionally, the device's software allows users to export the results in formats such as Excel files. The NanoDrop spectrophotometer covers a wavelength range of 200-800 nm. The NanoDrop spectrophotometer has applications in genomics, proteomics, drug discovery, molecular detection, and biomanufacturing.
In this laboratory, we utilize the Thermo Scientific NanoDrop 2000. The range of tests that can be conducted with laboratory devices:

  • Nucleic acid (Double and single stranded DNA / RNA)
  • Microarray analysis
  • UV-Vis spectroscopy
  • Protein A280 measurement
  • Proteins and label analysis
  • BCA protein assay
  • Lowry protein assay
  • Bradford protein assay
  • Pierce protein assay
  • Cell culture analysis
Basic and technical concepts of NanoDrop
The measurement of concentration is a crucial step in various experiments involving different samples. Without knowing the concentration of a substance dissolved in a solution, it is not possible to perform accurate tests on that sample. Spectrophotometry is an excellent technique for measuring the concentration of various substances in a solvent. This technique relies on the irradiation of the sample with light of a specific wavelength (in the ultraviolet and visible range) and then measuring the amount of light absorbed by the solution. Thermo Scientific NanoDrop 2000 utilizes a xenon lamp as its light source and a 2048-element linear silicon CCD array as its detector. This spectrophotometer offers a wide range of spectral coverage (190-850 nm) and can measure the concentration and quality of nucleic acids within the range of 2-3700 ng/ml without the need for sample dilution. It provides an optical absorption accuracy of 0.003 Abs.
Thermo Scientific NanoDrop 2000 offers several advantages, including the ability to measure cell density, indirectly measure protein mixture concentration by generating a standard curve, determine the concentration of cy-dye labels for microarray use, and automatically store all measurements separately based on absorbance per nanometer. Furthermore, it features a monitoring and troubleshooting system that allows users to assess the physical quality of the device's components. The spectrophotometer also incorporates error detection through warning signs and ensures result accuracy through internal sensor and digital photo analysis.
The table below presents a comparison of the optimal wavelengths utilized in various methods.





























Laboratory Assistant:
Leila Balaygoli (BSc in Laboratory Science); Email: l.goli @avicenna.ac.ir

Laboratory Substitute Assistant: Zhamak Pahlevanzadeh (MSc in Cellular and Molecular Biology); Email: j.pahlavanzadeh @avicenna.ac.ir;
Contact Number: +98 (21) 22432020 – Ext. 305
Location of Laboratory:The laboratory is situated on the second floor of Avicenna Research Institute, located within Shahid Beheshti University.
Working Schedule:The laboratory operates from Saturday to Wednesday, between 8:00 AM and 4:00 PM.
Service Policy and Costs: The cost of tests or services will vary depending on the sample type and specific testing requirements. Therefore, upon the initial inquiry, the laboratory will provide information regarding the cost of the requested test.

Sample Submission:To ensure smooth processing, please coordinate with the laboratory assistant at least 48 hours before sending the sample.


Method Sample spectrum
Nucleic acid 260 nm
UV visible 190-580 nm
Peptide 205 nm
Protein A280 280 nm
Lowry protein 650 nm
BCA protein 562 nm
Bradford protein 595 nm
Pierce protein 660 nm
Gold nanoparticles 520 nm
Cell culture 600 nm
Toxics 490 nm

Guide to use NanoDrop


پژوهشگاه فناوری های نوین علوم زیستی جهاد دانشگاهی - ابن سینا با هدف دستیابی به دانش فنی در زمینه فناوری‌های نوین زیستی از طریق انجام طرح‌های مطالعاتی و پژوهشی آزمایشگاهی و بالینی، از سال ۱۳۷۷ فعالیت خود را آغاز کرد.

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